Kirstenbosch National Botanical Garden
Walter Sisulu National Botanic Garden
Makana Botanical Gardens Eastern Cape
Free State National Botanical Garden Free State 1967
Johannesburg Botanical Garden Gauteng 1968
Pretoria National Botanical Garden Gauteng 1946
Manie van der Schijff Botanical Garden-University of Pretoria Gauteng 1934
Durban Botanic Gardens KwaZulu-Natal 1849
University of KwaZulu-Natal Botanical Garden KwaZulu-Natal 1983
KwaZulu-Natal National Botanical Garden KwaZulu-Natal 1874
Lowveld National Botanical Garden Mpumalanga 1969
North-West University Botanical Garden North West 1962
Hantam National Botanical Garden Northern Cape 2007
Karoo Desert National Botanical Garden Western Cape 1921
Harold Porter National Botanical Garden Western Cape 1959
Garden Route Botanical Garden Western Cape 1996
Stellenbosch University Botanical Garden Western Cape 1902 (since 1922 at current site)